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| Status |
Public on Mar 01, 2024 |
| Title |
KuLOs, septic, rep2 |
| Sample type |
SRA |
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| Source name |
human iPSC; M48
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| Organism |
Homo sapiens |
| Characteristics |
cell line: human iPSC; M48
cell type: human iPSC-derived liver organoids
genotype: WT
treatment: LPS/IFNr
time: Day 16
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from samples using the PureLink RNA Mini Kit (Thermo Fisher Scientific).
RNA libraries were prepared using 100 ng of total RNA with an Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
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| Description |
KuLO_sep_2
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| Data processing |
The sequencing reads were aligned to hg19_AmpliSeq_Transcriptome_ERCC_v1 using Torrent Mapping Alignment Program (TMAP).
QC metrics and normalized read counts per gene were obtained using AmpliSeqRNA plug-in v5.2.0.3, a Torrent Suite Software v5.2.2 (Thermo Fisher Scientific).
Assembly: hg19_AmpliSeq_Transcriptome_ERCC_v1
Supplementary files format and content: CSV files include read counts (RPM) for each Sample
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| Submission date |
May 25, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Yang Li |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Tokyo
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| Street address |
Shirokanedai 4-6-1
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| City |
Minato |
| State/province |
Tokyo |
| ZIP/Postal code |
1080071 |
| Country |
Japan |
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| Platform ID |
GPL17303 |
| Series (2) |
| GSE233483 |
A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction [KuLOs] |
| GSE233539 |
A human iPSC-derived multilineage liver organoid model containing Kupffer cells to study human sepsis-associated liver dysfunction |
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| Relations |
| BioSample |
SAMN35367939 |
| SRA |
SRX20517695 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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