|
| Status |
Public on Jul 06, 2011 |
| Title |
CUTLL1 wash2h+CHX_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CUTLL1 wash2h+CHX
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CUTLL1 (T lymphoblastic leukemia / lymphoma)
|
| Growth protocol |
CUTLL1 cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, penicillin and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared with RNeasy Mini kit (Qiagen) was subjected to expression profiling in triplicate on Affymetrix human genome U133 plus 2.0 arrays.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7-10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays.
|
| Scan protocol |
Arrays were scanned using a GenePix 4000B microarray scanner.
|
| Description |
Gene expression data from CUTLL1 cells treated with compound E for 3days and washouted compound E and treated with cycloheximide for 2 hours
|
| Data processing |
Affymetrix expression data were normalized using the standard robust multichip average (RMA) package in R.
|
| |
|
| Submission date |
May 26, 2011 |
| Last update date |
Jul 06, 2011 |
| Contact name |
James Zou |
| E-mail(s) |
[email protected]
|
| Phone |
6173010832
|
| Organization name |
Harvard University
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29544 |
Expression profiling of human T-LL cell line CUTLL1 |
|
