|
| Status |
Public on Apr 03, 2012 |
| Title |
Fresh tonsil, mRNA |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Nom-tumoral control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: fresh tonsil
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol reagent, purified by QIAGEN RNeasy columns and treated with DNase enzyme.
|
| Label |
Cy5
|
| Label protocol |
500ng RNA were used for cDNA and fluorescent cRNA synthesis. cRNA was purified and hybridized. All reagents and protocols were performed according to Agilent's recommendations.
|
| |
|
| Channel 2 |
| Source name |
Reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol reagent, purified by QIAGEN RNeasy columns and treated with DNase enzyme.
|
| Label |
Cy3
|
| Label protocol |
500ng RNA were used for cDNA and fluorescent cRNA synthesis. cRNA was purified and hybridized. All reagents and protocols were performed according to Agilent's recommendations.
|
| |
|
| |
| Hybridization protocol |
Hybridization settings: 17 hours, at 65ºC, at 10rpm. Blocking agent, hybridization and washing buffers was performed according according to Agilent's recommendations.
|
| Scan protocol |
Scan resolution 5um on an Agilent scanner.
|
| Description |
US22502553_251485019374_S01_GE2-v5_95_Feb07_1_2_FRESH_TONSIL
|
| Data processing |
Normalization type: lowess, quantiles, normexp (R program). Analysis: t-test (limma).
|
| |
|
| Submission date |
May 23, 2011 |
| Last update date |
Apr 03, 2012 |
| Contact name |
Lorena Di Lisio |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE23026 |
Combinatorial effects of microRNA to suppress the myc oncogenic pathway |
| GSE29492 |
Combinatorial effects of microRNA to suppress the myc oncogenic pathway (mRNA data) |
|
