|
| Status |
Public on Apr 03, 2012 |
| Title |
Burkitt Lymphoma12, miRNA |
| Sample type |
RNA |
| |
|
| Source name |
Tumoral sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: B-cell lymphoma
tumor type: Burkitt lymphoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol reagent (Invitrogen, Carlsbad, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were dephosphorylated, denaturalizated, treated with ligase T4 and labelled with Cy3 for 2 hours at 16ºC.
|
| |
|
| Hybridization protocol |
Hybridization settings: 20 hours, at 55ºC, at 20rpm. Blocking agent, hybridization and washing buffers was performed according to Agilent's recommendations.
|
| Scan protocol |
Scan resolution 5um on an Agilent scanner.
|
| Description |
251643612182_S01_miRNA-v1_95_May07_2_3_BL
Corresponds to GSM568278
|
| Data processing |
Limma package was used to import the data. After between-array median normalization by the R program, significant analysis of microarray (SAM) Excel macro was used.
|
| |
|
| Submission date |
May 23, 2011 |
| Last update date |
Apr 03, 2012 |
| Contact name |
Lorena Di Lisio |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8617 |
| Series (2) |
| GSE23026 |
Combinatorial effects of microRNA to suppress the myc oncogenic pathway |
| GSE29491 |
Combinatorial effects of microRNA to suppress the myc oncogenic pathway (miRNA data) |
|
| Relations |
| Reanalysis of |
GSM568278 |
