|
| Status |
Public on Aug 01, 2011 |
| Title |
Human Exocervix Female 9 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy female exocervix
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ssDNA were hybridized for 16 hr at 45C and 60 rpm on Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 protocol FS450_0007
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) with GeneChip Scanner 3000
|
| Description |
RMA default expression value derived from Affymetrix Expression Console software
|
| Data processing |
Microarrays were normalized using the RMA algorithm (Robust Multichip Average) in the Affymetrix Expression Console. The analysis was done at Gene Level Default: RMA-Sketch
probe group file: HuGene-1_0-st-v1.r4.pgf
meta-probeset file: HuGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
May 11, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Jaime Berumen |
| E-mail(s) |
[email protected]
|
| Phone |
+52(55)27892000
|
| Organization name |
Universidad Nacional Autonoma de Mexico
|
| Department |
Departamento de Medicina Experimental
|
| Lab |
Unidad de Medicina Genómica
|
| Street address |
Dr. Balmis #148 Col. Doctores
|
| City |
Mexico City |
| ZIP/Postal code |
06726 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE29216 |
Amplified genes are not necessarily over expressed, they can be unchanged or down regulated in cervical cancer cell lines [expression array data] |
| GSE29245 |
Amplified genes are not necessarily over expressed, they can be unchanged or down regulated in cervical cancer cell lines |
|
