|
| Status |
Public on May 11, 2011 |
| Title |
S6-/-_24 hrs |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Embryonic Fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Sirt6 -/-
treatment: TNF-α for 24 hrs
cell type: MEF
|
| Treatment protocol |
Cells were treated with TNF-α (20 ng/mL) for the indicated times.
|
| Growth protocol |
MEFs were generated from 13.5-day-old embryos using standard methods and propagated in DMEM (Invitrogen) plus 15% FBS. MEFs were passaged a total of four times before TNF-α treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol (Invitrogen) from mouse embryonic fibroblasts. RNA was labeled with Cy5 and hybridized to whole genome mouse bead arrays (Illumina).
|
| Label |
Cy5
|
| Label protocol |
Cy5 cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
rank invariant normalization performed by Illumina's GenomeStudio software
|
| |
|
| Submission date |
Apr 13, 2011 |
| Last update date |
May 11, 2011 |
| Contact name |
Kun Qu |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Lab |
Howard Chang
|
| Street address |
269 Campus Dr. CCSR 2150
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE28585 |
Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks (Illumina) |
| GSE28641 |
Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks |
|
