|
| Status |
Public on Feb 25, 2023 |
| Title |
T3 |
| Sample type |
RNA |
| |
|
| Source name |
hypertrophic scar
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hypertrophic scar
agent: glucocorticoid triamcinolone acetonide mixed with lidocaine
|
| Treatment protocol |
These patients were diagnosed by routine pathological examination and were given local injections of glucocorticoid triamcinolone acetonide mixed with lidocaine in for more than 3 months. Patients who had received lidocaine treatment were enrolled as controls. Patients received surgical treatment to resect the HS tissues.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Patients received surgical treatment to resect the HS tissues. Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific) and the RNA integrity(RIN) was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were dephosphorylated, denaturated and then labeled with Cyanine-3-CTP.
|
| |
|
| Hybridization protocol |
After purification the labeled RNAs were hybridized onto the microarray.
|
| Scan protocol |
After washing, the arrays were scanned with the Agilent Scanner G2505C (Agilent Technologies).
|
| Description |
microRNA expression after triamcinolone acetonide and lidocaine injection
|
| Data processing |
Feature Extraction software (version10.7.1.1, Agilent Technologies) was used to analyze array images to get raw data.
Next, the raw data was normalized with the quantile algorithm. The probes that at least 100.0 percent of samples in any 1 condition out of 2 conditions have flags in Detected were chosen for further data analysis. Differentially expressed miRNAs were then identified through fold change as well as P value calculated using t-test. The threshold set for up- and down-regulated genes was a fold change >= 2.0 and a P value <= 0.05.
|
| |
|
| Submission date |
Feb 20, 2023 |
| Last update date |
Feb 26, 2023 |
| Contact name |
huayu Zhu |
| E-mail(s) |
[email protected]
|
| Organization name |
Fourth
|
| Street address |
No. 28 Road
|
| City |
Xi'an |
| ZIP/Postal code |
710049 |
| Country |
China |
| |
|
| Platform ID |
GPL24741 |
| Series (1) |
| GSE225646 |
Glucocorticoid promotes fatty acids metabolism in hypertrophic scar tissues and fibroblasts via the microRNA |
|
