|
| Status |
Public on Apr 09, 2011 |
| Title |
Adrenocortical adenoma with wild-type CTNNB1-2 |
| Sample type |
RNA |
| |
|
| Source name |
WT AA 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Adrenocortical adenoma
tumor secretion profile: Cortisol
genotype: WT CTNNB1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 GeneCHIP.
|
| Scan protocol |
GeneChips were scanned using the Genechip scanner 3000 workstation (Affymetrix)
|
| Description |
Gene expression profile of an adrenocortical adenoma with wild-type CTNNB1 gene
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 09, 2011 |
| Contact name |
julien durand |
| E-mail(s) |
[email protected]
|
| Phone |
514-890-8000
|
| Organization name |
CRCHUM
|
| Department |
Biomedicale Science
|
| Street address |
3840 Rue Saint-Urbain
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H2W 1T8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28476 |
Characterization of differential gene expression in adrenocortical tumors harbouring β-catenin (CTNNB1) mutations. |
|
