|
| Status |
Public on Feb 11, 2023 |
| Title |
long_RNA_w GLKD 3-5h progeny biol rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
embryo
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: embryo
genotype: w GLKD progeny
treatment: none
|
| Growth protocol |
Flies were raised at 25°C on cornmeal medium. Embryos were collected on same medium plus fresh yeast.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100 ul of embryos using TRIzol (Life Technologies) and 1 µg was treated with DNase (Fermentas).
Libraries were prepared using the RNA RiboZero Stranded protocol by Fasteris.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
A DeSEq2 analyze on all D. mel. genes (release 6.36) was performed using the two biological replicates for each genotype, control or Kdm3 GLKD progeny.
Assembly: dm6_clean
Supplementary files format and content: bigWig, DEseq2 result for biological triplicates as a tab-delimited text file
|
| |
|
| Submission date |
Feb 10, 2023 |
| Last update date |
Feb 11, 2023 |
| Contact name |
Antoine Boivin |
| E-mail(s) |
[email protected]
|
| Organization name |
Sorbonne Universite CNRS
|
| Department |
IBPS
|
| Lab |
LBD UMR7622
|
| Street address |
9 quai St Bernard
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE203279 |
The histone demethylase KDM3 prevents auto-immune piRNAs production in Drosophila |
|
| Relations |
| BioSample |
SAMN33242345 |
| SRA |
SRX19331311 |
