|
| Status |
Public on Oct 01, 2011 |
| Title |
DF508 1 |
| Sample type |
RNA |
| |
|
| Source name |
IB3 transfected with DF508
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IB3-1
cell type: Bronchial epithelial cell line
transfection: CFTR DF508 mutant
|
| Treatment protocol |
CFTR and mutated forms were subcloned into the mammalian expression vector, pCDNA 3.1 and transfected into cells using Lipofectamine 2000 (Invitrogen)
|
| Growth protocol |
IB3-1 cells (CF genotype _F508/W1282X, bronchial epithelial derivation), were grown in LHC-8 Basal Medium, 95%; fetal bovine serum, 5%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr on GeneChip the U133 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the AffyMetrix GeneChip Scanner
|
| Description |
Gene expression data IB3 cells transfected with DF508 mutant of CFTR.
|
| Data processing |
Microarray scans were processed in GCOS 1.2 to determine expression levels using Affymetrix default analysis settings and global scaling as normalization method.The trimmed mean target intensity of each array was set to 100.
|
| |
|
| Submission date |
Mar 21, 2011 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Jerry M. Wright |
| E-mail(s) |
[email protected]
|
| Phone |
410-614-5010
|
| Organization name |
Johns Hopkins Medical Institutions
|
| Department |
Physiology
|
| Street address |
725 N. Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE28072 |
Expression data from transfected IB3 cells transfected with CFTR mutants |
|
