|
| Status |
Public on Dec 06, 2023 |
| Title |
TMD8-#2 |
| Sample type |
SRA |
| |
|
| Source name |
TMD8
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TMD8
cell type: DLBCL
genotype: Ibrutinib resistant clone
treatment: Ibrutinib (10nM)
|
| Treatment protocol |
Cells were treated with Ibrutinib (10-20nM) for a year. Resistant clones were generated by serial dilution at 1:3 ratio. Resistant phenotype were confirmed by treat cells with ibrutinib compared to parental cell lines
|
| Growth protocol |
DLBCL cells were grown in RPMI1640 media plus 20%FBS,1% penicillin, 1% streptomycin.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total mRNA was extracted by Rneasy plus mini kit (Qiagen) following the manufacturer’s instructions.
cDNA library was construbuted by using Illumina Truseq RNA Library Prep Kit (RS-122-2002).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
FPKM-TMD8.txt
|
| Data processing |
BAM file were generated by STAR-2.5.2b;picard to remove duplicated reads
RNAseq fpkm were generated by HISAT, StringTie and Ballgown by following Pertea et al.(2016).
Assembly: hg19
Supplementary files format and content: RNA-seq (txt file: gene fpkm)
|
| |
|
| Submission date |
Jan 18, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Lixin Rui |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Medicine
|
| Lab |
Lixin Rui
|
| Street address |
1111 Highland Ave R.4060
|
| City |
Madison |
| State/province |
WI - Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN32782269 |
| SRA |
SRX19061642 |
