|
| Status |
Public on Jul 01, 2011 |
| Title |
Bmpr2-Delx4 1 |
| Sample type |
RNA |
| |
|
| Source name |
PMVEC isolated from mice carrying TetO7-Bmpr2-delx4, immortomouse and Rosa26-rtTA transgenes
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N
genotype/variation: Bmpr2-delx4+ mutation
cell type: pulmonary vascular microendothelial cells (PMVEC)
|
| Treatment protocol |
Full serum, not confluent
|
| Growth protocol |
Normoxic PMVEC, approximately 50% confluent in 10% serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from freshly cultured PMVEC using a Qiagen RNEasy kit
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Manual)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Manual)
|
| Scan protocol |
Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Manual)
|
| Description |
Delx4-1
|
| Data processing |
Data was normalized (RMA) using Expression Console
|
| |
|
| Submission date |
Mar 18, 2011 |
| Last update date |
Jul 01, 2011 |
| Contact name |
James West |
| E-mail(s) |
[email protected]
|
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Pulmonary
|
| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
