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Sample GSM692736 Query DataSets for GSM692736
Status Public on Sep 30, 2011
Title siControl treated with E2 rep1
Sample type RNA
 
Source name MCF7 breast cancer cells
Organism Homo sapiens
Characteristics cell line: MCF7 breast cancer cells
era status: positive
genotype/variation: control
agent: estradiol (E2)
Treatment protocol 72h post transfection with the appropriate siRNA, cells were stimulated with 10nM E2 or DMSO for 3h prior RNA extraction
Growth protocol Cells were grown in stripped media (DMEM w/o phenol, CDT treated serum 10%, glutamine and penicillin/streptomycin).
Extracted molecule total RNA
Extraction protocol RNA was extracted with with QIAGEN RNeasy kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Efficient knock down was validated with RT-qPCR.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Control_E1
replicate 1
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Mar 17, 2011
Last update date Sep 30, 2011
Contact name luca magnani
E-mail(s) [email protected]
Phone 6036539932
Fax 6036539952
Organization name Dartmouth College
Department Genetics
Lab Lupien
Street address NCCC one medical drive
City Lebanon
State/province NH
ZIP/Postal code 03756
Country USA
 
Platform ID GPL10558
Series (2)
GSE28006 The pioneer factor PBX1 guides a distinct ERa signaling in breast cancer [mRNA profiling]
GSE28008 The pioneer factor PBX1 guides a distinct ERa signaling in breast cancer

Data table header descriptions
ID_REF
VALUE quantile normalized signal

Data table
ID_REF VALUE
ILMN_1762337 6.558604717
ILMN_2055271 6.99328661
ILMN_1736007 6.413479805
ILMN_2383229 6.755509377
ILMN_1806310 6.483787537
ILMN_1779670 6.276833534
ILMN_1653355 6.59536314
ILMN_1717783 6.032907963
ILMN_1705025 6.684782028
ILMN_1814316 6.550827503
ILMN_2359168 5.741911888
ILMN_1731507 5.654619694
ILMN_1787689 6.219118118
ILMN_3241953 7.083380222
ILMN_1745607 5.736436844
ILMN_2136495 5.63415575
ILMN_1668111 6.561704159
ILMN_2295559 6.321399212
ILMN_1735045 8.40918541
ILMN_1680754 6.179316998

Total number of rows: 47231

Table truncated, full table size 1147 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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