|
| Status |
Public on May 06, 2011 |
| Title |
HSC old female , biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HSC old female
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl6
genotype: wild type
tissue: Mouse bone marrow
age: 24 months
gender: female
|
| Growth protocol |
Primary Cells directly sorted for RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed with QIAGEN microRNeasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4000 to 7000 cell
|
| |
|
| Hybridization protocol |
RNA hybridized to MOE430_2. Chips according to manufacturer manual
|
| Scan protocol |
GeneChips were scanned using the GeneChipTM Scanner 3000
|
| Data processing |
Probe level expression values and normalization were calculated using RMAExpress and further analysis was done using dChip
|
| |
|
| Submission date |
Mar 04, 2011 |
| Last update date |
May 06, 2011 |
| Contact name |
Gudmundur Norddahl |
| Organization name |
Lund University
|
| Street address |
Tornavagen 10
|
| City |
Lund |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27686 |
Accumulating mitochondrial DNA mutations drive premature hematopoietic aging phenotypes molecularly distinct from physiologic stem cell aging |
|
