|
| Status |
Public on Feb 17, 2023 |
| Title |
CD34+CD45RA-CD90+ scRNAseq, edited by AAVS1, day 3 post-nucleofection |
| Sample type |
SRA |
| |
|
| Source name |
HSPC
|
| Organism |
Homo sapiens |
| Characteristics |
cell markers: CD34+CD45RA-CD90+ cells
cell type: HSPC
genotype: AAVS1
time: Day 4
|
| Treatment protocol |
CD34+ cells were nuclefected by AAVS1 or MYSM1 CRISPR RNPs
|
| Growth protocol |
Immobilized bone marrow CD34+ cells were cultured in CD34+ cell expansion medium with Stemcell Technology's serum free expansion medium as base medium supplemented with 1X cc100 supplement, 100ng/ml TPO and 35nM UM171
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
CD34+CD45RA-CD90+ cells were FACS sorted and 7000 cells were used in 10Xgenomics single cell RNA seq library preparation kit following manufacturer's protocols exactly
library constructed using 10Xgenomics single cell RNA seq library construction kit following manufacturer's instruction
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Cellranger
Sequence reads were analyzed by 10Xgenomics software Cellranger with default setting
matrix file was used for downstream differential gene expression analysis by Seurat package in R
Assembly: hg38
Supplementary files format and content: matrix files, barcode files, feature files
|
| |
|
| Submission date |
Dec 16, 2022 |
| Last update date |
Feb 17, 2023 |
| Contact name |
Jiawei Zhao |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston Children's Hospital
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE221169 |
Human Hematopoietic Stem Cell Vulnerability to Ferroptosis [scRNA-seq] |
| GSE221170 |
Human Hematopoietic Stem Cell Vulnerability to Ferroptosis |
|
| Relations |
| BioSample |
SAMN32275916 |
| SRA |
SRX18728758 |
