|
| Status |
Public on Nov 16, 2013 |
| Title |
Muscle biopsies from sedentary COPD patient #27 with normal Body Mass Index |
| Sample type |
RNA |
| |
|
| Source name |
Sedentary COPD with normal BMI
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: musculus vastus lateralis
disease state: chronic obstructive pulmonary disease (COPD)
exercise state: sedentary
bmi: normal
individual: 27
|
| Treatment protocol |
8 weeks of exercise training.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy extraction kit (QIAGEN, USA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Amplification and labeling of cRNA was performed with the Affymetrix IVT labeling kit.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip technology was performed using Human U133 Plus2 GeneChips according to the manufacturer's suggested protocols.
|
| Scan protocol |
Affymetrix Scanner 3000 7G.
|
| Description |
Gene expression data from muscle biopsies of a sedentary COPD individual with normal BMI.
|
| Data processing |
The data were quantile normalized using the R package RMA.
|
| |
|
| Submission date |
Feb 25, 2011 |
| Last update date |
Nov 16, 2013 |
| Contact name |
Nil Turan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Birmingham
|
| Street address |
University of Birmingham, Edgbaston
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27536 |
Vastus lateralis biopsies from healthy and COPD patients before and after 8 weeks of exercise training |
|
