Primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 embryos bearing one conditional knockout allele of p300 and were infected at passage 3 with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated under normoxia with 5% carbon dioxide at 37 C in a humid chamber with 100ng/ml Trichostatin A (TSA), then with 100um dipyridyl (DP) after 30min. 3 hrs later, cells were lysed in Trizol for RNA extraction. Sample Type: Mouse Total RNA Project: Brindle Sample User: jmorris Probe Array Type: Mouse430_2 Barcode: @52002900572820040206400907920925 Probe Array Lot: 4009079 Expiration Date: Apr 02 2006 12:00AM Genus: species: Strain/Line: Individual: Sample Provider: Sex: Age: Developmental State: Tissue: Cell Type: Genetic Modifications: Genetic Characteristics: Disease State: Reference to Clinical Data: Growth Conditions: in vivo Treatments: Tissue Harvesting Method: Extract Method: Molecule Type: Amplification: Amount: Label: Label Incorporation: Lab Protocol: Affy Protocol Experiment User: jmorris Algorithm: Statistical HZ:4 VZ:4,BG:2,SmoothFactorBG:100,Epsilon:0.5,BF:,Alpha1:0.05,Alpha2:0.065,Tau:0.015,Gamma1H:0.0025,Gamma1L:0.0025,Gamma2H:0.003,Gamma2L:0.003,Perturbation:1.1,TGT:500,NF:1.000000000000,SF:4.466100215912,SFGene:All Background:Avg:102.05,Stdev:2.01,Max:107.8,Min:96.9 Noise:Avg:6.89,Stdev:0.27,Max:7.8,Min:6.3 RawQ:3.64 Corner+:Avg:476,Count:32 Corner-:Avg:9501,Count:32 Central-:Avg:10469,Count:9
Two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression
Data table header descriptions
ID_REF
As defined by Affymetrix, these are the probe set identifiers, each of which is unique to a specific probe set defining a specific region of a single gene or set.
VALUE
This is the final calculated meauremeent for each probe set identifer that has been made comparable, through scaling or normalization, accross all samples and rows.
DETECTION P-VALUE
Defines the confidence in the calculated signal accurately reflecting the expression level of the specific gene or est that the probe set identifies.
