|
| Status |
Public on Feb 12, 2011 |
| Title |
M5 Patient No.5, bone marrow sample |
| Sample type |
genomic |
| |
|
| Source name |
Bone Marrow 5 at diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
tumor: Acute monocytic leukemia
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37oC using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
standard Illumina protocol standard Illumina protocol
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
| Description |
Genomic DNA extracted from leukemia bone marrow blast cells was genotyped using Human660W-Quad v1.0 Genotyping BeadChips (Illumina).
|
| Data processing |
Genomic DNA extracted from blood was genotyped using Genotyping BeadChips (Illumina). Image data was analyzed using Genomestudio v2010.2 with GT module 1.6.3 (Illumina).
|
| |
|
| Submission date |
Feb 09, 2011 |
| Last update date |
Feb 12, 2011 |
| Contact name |
Zhaohui Gu |
| E-mail(s) |
[email protected]
|
| Organization name |
Shanghai Jiao Tong University
|
| Department |
School of Medicine
|
| Lab |
State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology
|
| Street address |
197 Rui Jin Road II
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL8888 |
| Series (2) |
| GSE27193 |
Normalization of whole-genome SNP data from acute monocytic leukemia patients for exome sequencing |
| GSE27244 |
Expression profile and whole genome SNP data from acute monocytic leukemia patients |
|
