|
| Status |
Public on Feb 01, 2011 |
| Title |
normal arterial tissue: sample #1 |
| Sample type |
RNA |
| |
|
| Source name |
normal superficial temporal artery
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: arterial
status: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
sample collection protocol: The arterial tissues were snap-frozen in liquid nitrogen, and stored at –80 ° C.
Total RNA was isolated using TRIzol (Invitrogen) and cleaned using RNeasy kit (Qiagen) following the manufacturers’ protocol. The integrity of total RNA was confirmed by agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
Labeled cRNA was synthesized from cDNA using the GeneChip IVT labeling kit (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Fragmented cRNA were hybridized according to the standard protocol in the GeneChip® Expression Analysis Technical Manual.
|
| Scan protocol |
Arrays were scanned using the Affymetrix® GeneChip® Scanner 3000.
|
| Description |
Gene expression data
|
| Data processing |
The data were analyzed with whole chip Robust Multi-chip Average (RMA) analysis.
|
| |
|
| Submission date |
Jan 31, 2011 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Fan Jiang |
| E-mail(s) |
[email protected]
|
| Phone |
+86 531 8216 9267
|
| Organization name |
Shandong University
|
| Street address |
107 Wen Hua Xi Road
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26969 |
Transcriptome-wide characterization of gene expression associated with unruptured intracranial aneurysms |
|
