|
| Status |
Public on Jan 26, 2011 |
| Title |
P19, biological rep4 (MOE430A) |
| Sample type |
RNA |
| |
|
| Source name |
P19 mouse embryonal carcinoma cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C3H
gender: male
tissue: embryo
cell line: P19 mouse embryonal carcinoma cells
|
| Growth protocol |
The mouse embryonal carcinoma cells were maintained in alpha-minimum essential medium (Invitrogen) supplemented with 10% fetal calf serum (FCS, Cell Culture Technologies), 2 mM L-glutamine (Sigma-Aldrich), 100 U/ml penicillin, and 100 µg/ml streptomycin (Invitrogen) at 37°C in an atmosphere containing 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells with an RNeasy Mini kit (QIAGEN)according to the manufacturer’s instruction.
|
| Label |
biotin
|
| Label protocol |
Total RNA isolated from undifferentiated EC cell strains (P19 cells, P19CL6 cells, and 4 P19CL6 sublines) was converted to biotinylated cRNA using a Two-Cycle Target Labeling and Control Reagents (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled RNA was processed for microarray hybridization to MOE430A GeneChips (Affymetrix). An Affymetrix GeneChip Fluidics Station was used to perform streptavidin/phycoerythrin staining.
|
| Scan protocol |
The hybridization signals on microarray were scanned using a GeneChip Scanner 3000 (Affymetrix)
|
| Description |
Gene expression data from a mouse embryonal carcinoma cell strain
|
| Data processing |
The data were analyzed with GCOS software. Normalization was performed by global scaling with the arrays scaled to a trimmed average intensity of 500 after excluding the 2% of probe sets with the highest and the lowest values.
|
| |
|
| Submission date |
Jan 26, 2011 |
| Last update date |
Jan 26, 2011 |
| Contact name |
Yoji Sato |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3700-1141
|
| Fax |
+81-3-3707-6950
|
| Organization name |
NIHS
|
| Department |
Cell. Gene Thpy. Prod.
|
| Street address |
1-18-1 Kami-yoga
|
| City |
Setagaya |
| State/province |
Tokyo |
| ZIP/Postal code |
158-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE26875 |
Gene expression data from undifferentiated mouse embryonal carcinoma cell strains (MOE430A and MOE430B) |
|
