|
| Status |
Public on Jun 24, 2011 |
| Title |
A549 cells_24h SIS3_biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells, 24h SIS3, biological rep1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung epithelial cells
gender: male
age: 58y
|
| Treatment protocol |
Cultured A549 cells treated with DMSO 30 min prior to stimulation with 2ng/ml TGF-b
|
| Growth protocol |
Cells grown in F12-K culture medium supplemented with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 500ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 60C on Agilent 4X44K gene expression array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
|
| Description |
Cultured A549 cells treated with 10uM SIS3 30 min prior to stimulation with 2ng/ml TGF-b
|
| Data processing |
Agilent Feature Extraction v9.5.2, default parameters
|
| |
|
| Submission date |
Jan 25, 2011 |
| Last update date |
Jun 24, 2011 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
[email protected]
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE26858 |
SMAD3 targets in A549 lung epithelial cells |
|
