disease state: gastric cancer gender: male tissue: blood tsi, raw drug sensitivity: 39 tsi.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 fu, raw drug sensitivity: 36 fu.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 oxa, raw drug sensitivity: 30 oxa.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 dtax, raw drug sensitivity: 3 dtax.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 1 cdp, raw drug sensitivity: 3 cdp.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 1 tax, raw drug sensitivity: 40 tax.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 saha, raw drug sensitivity: 49 saha.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 pxd, raw drug sensitivity: 43 pxd.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 2 cg2, raw drug sensitivity: 8 cg2.30, binary data (i.e. >= 30 or < 30) at 30% cutoff: 1
Extracted molecule
genomic DNA
Extraction protocol
Genomic DNA was extracted from blood samples using the Qiagen DNeasy Kit according to standard instructions.
Label
biotin
Label protocol
Standard Affymetrix protocol (The fragmented DNA was labeled using DNA Labeling Reagent and TdT enzyme for 4.25 hours at 37℃.)
Hybridization protocol
Standard Affymetrix protocol (The labeled DNA was hybridized to a Genomic-Wide Human SNP Array 6.0 for 16-18 hours in a rotating oven at 50℃ and 60 rpm.)
Scan protocol
Standard Affymetrix protocol (The SNP Array 6.0 was scanned with the Affymetirx GeneChip Scanner 3000 using Affymetrix GeneChip Operating Software (GCOS; version 1.4))
Data processing
Standard Affymetrix protocol (Signal intensity data was analyzed by the Dynamic Model (DM) algorithm. A contrast QC of greater than or equal to 0.4 should be used to determine whether a sample should be repeated or used for downstream ananlysis.) Standard Affymetrix protocol (Genotypes were called by the Affymetrix Genotyping Console Software (GTC; version 3.0.1) based on the Birdseed version 2 algorithm.
