|
| Status |
Public on May 23, 2011 |
| Title |
HoxB4_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
c-Kit+CD41+ cells/HoxB4
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hematopoietic stem/progenitor cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
A master mix using the 5X TdT Buffer, TdT and DNA Labeling reagent can be prepared just before aliquoting 15 μL into the 0.2 mL strip tubes containing the 45 μL of Fragmented Single-Stranded DNA. Incubate the reactions at 37°C for 60 minutes, 70°C for 10 minutes and 4°C for at least 2 minutes
|
| |
|
| Hybridization protocol |
Heat the Hybridization Cocktail at 99°C for 5 minutes. Cool to 45°C for 5 minutes. Inject the appropriate amount of the specific sample into the GeneChip ST array through one of the septa. Place array in 45°C hybridization oven, at 60 rpm, and incubate for 17 hours ± 1 hour. Please refer to the GeneChip® Expression Wash, Stain and Scan User Manual (P/N 702731) for the washing and staining steps required immediately after completion of hybridization.
|
| Scan protocol |
GeneChip Scaner 3000 was used . The core software was used AGCC for image processing.
|
| Description |
c-Kit+CD41+ hematopoietic progenitor cells transduced with a HoxB4 control retrovirus cocultured with OP9 stromal cells for 8 days in the absence of doxycyclin
|
| Data processing |
The summarized probe set data were generated using Expression console. RMA-sketch was used as the processing algorithm.
|
| |
|
| Submission date |
Jan 14, 2011 |
| Last update date |
May 23, 2011 |
| Contact name |
Takaho A. Endo |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Laboratory for Integrative Genomics
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26653 |
HoxB4 in hematopoietic stem cells |
|
