|
| Status |
Public on May 27, 2011 |
| Title |
Fezf1-/- 1 |
| Sample type |
RNA |
| |
|
| Source name |
FACS isolated Fezf1 expressing cells from Fezf1-/- MOE
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Fezf1 -/-
age: E18.5
cell type: Fezf1 -/- cells from Main Olfactory Epithelium
genetic background: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plus Micro Kit according to manufacture's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip Whole Transcript (WT) Sense Target Laebeling Assay Manuel Version 4, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled cRNA were fragmented and hybridized according to the standard Affymetrix protocol (GeneChip Whole Transcript (WT) Sense Target Laebeling Assay Manuel Version 4, 2007, Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
GC-RMA normalization using Affymetrix Expression Console
|
| |
|
| Submission date |
Dec 14, 2010 |
| Last update date |
May 27, 2011 |
| Contact name |
Matthew Joseph Eckler |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSC
|
| Department |
MCDB
|
| Lab |
Bin Chen
|
| Street address |
434 Sinsheimer Labs, UCSC
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26064 |
Gene Expression Analysis of Fezf1 Mutant Main Olfactory Epithelium |
|
