|
Status |
Public on Oct 26, 2014 |
Title |
control3_v1_poly(I:C) |
Sample type |
RNA |
|
|
Source name |
whole blood from healthy control
|
Organism |
Homo sapiens |
Characteristics |
gender: F race: White illness: healthy control geographic: France age at enrollment: 34 cell population: Whole blood in vitro stimulation condition: poly(I:C) in vitro stimulation incubation time: 2h in vitro stimulation blood volume per condition: 1 ml
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the MagMAX™-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion, Austin, TX, USA) according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were generated following manufacturer protocols using the Illumina TotalPrep Amplification Kit from Ambion
|
|
|
Hybridization protocol |
Hybridization to HumanHT-12 v4 Expression BeadChip
|
Scan protocol |
SentrixScanVersion 2.3.0.13
|
Description |
Set1
|
Data processing |
The data were analysed using BeadStudio version 1.5.1.3 using the average chip normalization method.
|
|
|
Submission date |
Dec 01, 2010 |
Last update date |
Oct 26, 2014 |
Contact name |
Damien Chaussabel |
E-mail(s) |
[email protected]
|
Organization name |
Baylor Institute for Immunology Research
|
Street address |
3434 Live Oak
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75204 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE25742 |
Genome-wide profiling of whole blood from patients with defects in Toll-like receptors (TLRs) and IL-1Rs (the TIR pathway) signaling |
|