|
| Status |
Public on Oct 26, 2014 |
| Title |
IRAK4 P3_LPS |
| Sample type |
RNA |
| |
|
| Source name |
whole blood from IRAK4-/- patient
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
race: White
illness: IRAK4-/- patient
geographic: Portugal
age at enrollment: 14
cell population: Whole blood
in vitro stimulation condition: LPS
in vitro stimulation incubation time: 2h
in vitro stimulation blood volume per condition: 1 ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the MagMAX™-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion, Austin, TX, USA) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were generated following manufacturer protocols using the Illumina TotalPrep Amplification Kit from Ambion
|
| |
|
| Hybridization protocol |
Hybridization to HumanHT-12 v4 Expression BeadChip
|
| Scan protocol |
SentrixScanVersion 2.3.0.13
|
| Description |
Set2
|
| Data processing |
The data were analysed using BeadStudio version 1.5.1.3 using the average chip normalization method.
|
| |
|
| Submission date |
Dec 01, 2010 |
| Last update date |
Oct 26, 2014 |
| Contact name |
Damien Chaussabel |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor Institute for Immunology Research
|
| Street address |
3434 Live Oak
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE25742 |
Genome-wide profiling of whole blood from patients with defects in Toll-like receptors (TLRs) and IL-1Rs (the TIR pathway) signaling |
|
