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Sample GSM626969 Query DataSets for GSM626969
Status Public on Nov 23, 2010
Title CD8 KLF13 KO 1
Sample type RNA
 
Source name CD8 KLF13 KO
Organism Mus musculus
Characteristics strain: BALB/c
cell type: CD8 T
genotype/variation: KLF13 KO
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with RNAeasy kit from Qiagen RNeasy. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
Label biotin
Label protocol 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer's protocol.
 
Hybridization protocol Four replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse Gene ST 1.0 GeneChip
Scan protocol scanned on Affymetrix GeneChip scanner 3000. Data were collected using Affymetrix GCOS software.
Data processing Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm. Differentially expressed genes were identified with ANOVA analysis. Genes that are up- or down-regulated more than 2 fold and with a p<0.001 were considered significant. Significant genes were analyzed for enrichment for pathways using DAVID bioinformatics database (http://david.abcc.ncifcrf.gov/) and Ingenuity Pathway Analysis software.
 
Submission date Nov 19, 2010
Last update date Nov 22, 2010
Contact name Dazhi Lai
Organization name NIH
Department NHLBI
Lab LMI
Street address 10 Center Dr, 7B20A
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL6246
Series (1)
GSE25502 KLF13 regulate memory-like CD8 T cells

Data table header descriptions
ID_REF
VALUE rma normalized

Data table
ID_REF VALUE
10338001 11.1736
10338002 6.00088
10338003 9.39914
10338004 8.57175
10338005 1.95143
10338006 2.21601
10338007 2.5085
10338008 3.00008
10338009 8.31709
10338010 2.02148
10338011 5.17139
10338012 2.07541
10338013 1.82457
10338014 1.8913
10338015 1.87015
10338016 7.41967
10338017 12.2916
10338018 6.48166
10338019 4.46452
10338020 8.30316

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM626969.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM626969.chp.gz 269.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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