|
| Status |
Public on Jul 01, 2022 |
| Title |
TWCM-761 |
| Sample type |
SRA |
| |
|
| Source name |
Transmural LV Apex
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Transmural LV Apex
storage: minus 80 degrees
technology: Single Nuclei
disease state: Sarcoidosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Transmural tissue samples were removed from the left ventricle apex during LVAD implantation or after whole heart explantation. Samples for single nuclei were frozen in liquid nitrogen and stored at -80C until use and processed by mechanical homogenization. All single nuclei suspensions were sorted by FACS to remove debris.
10X Genomics Chromium Single Cell 5’ Reagent V1.1
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
Alignment: Cell Ranger V3, aligned to GRCh38 transcriptome, or custom pre-mrna reference.
QC and Filtering: Seurat V3/V4
Unsupervised Clustering: Seurat V3/V4
Assembly: GRCh38 V3.0.0
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jun 08, 2022 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Pan Ma |
| E-mail(s) |
[email protected]
|
| Phone |
3149306214
|
| Organization name |
Washington University in Saint Louis
|
| Street address |
CSRB, Room 9918, 425 S Euclid, Saint Louis, MO, 63110
|
| City |
Saint Louis |
| State/province |
United States |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE205734 |
Transcriptional and immune landscape of cardiac sarcoidosis |
|
| Relations |
| BioSample |
SAMN28928302 |
| SRA |
SRX15643389 |
