|
| Status |
Public on Jan 15, 2012 |
| Title |
Skov3 Flag P10 |
| Sample type |
RNA |
| |
|
| Source name |
Flag plasmid, passage #10
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKOV-3 cells
cell passage: P10
transfectant: Flag plasmid
|
| Treatment protocol |
SKOV-3 cells were seeded one day before transfection with pCMV-Flag-Evi1 plasmid, pCMV-Flag plasmid, Evi1 siRNA (Evi1 ON-TARGETplus siRNA#8 plus #10) or control siRNA (ON-TARGETplus Non-targeting Pool, Dharmacon)
|
| Growth protocol |
SKOV-3 cells (ATCC, HTB-77) were grown at 37degres in 10%FBS RPMI. They were passaged every 3-4 days. P8, P9, P10, P11 in the sample names refer to the cell passage number.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared with GeneChip WT cDNA synthesis and Amplification Kit, GeneChip WT terminal Labeling Kit and GeneChip Sample Cleanup Module (Affymetrix).
|
| |
|
| Hybridization protocol |
GeneChip Hybridization Control Kit and GeneChip Hybridization, Wash, Stain Kit (Affymetrix) were used to hybidize Human Gene 1.0ST arrays.
|
| Scan protocol |
Standard Affymetrix Scan protocol was followed.
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.4 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Justin Jeyakani Joseph Gnanakkan |
| E-mail(s) |
[email protected]
|
| Phone |
68088173
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Computational and Systems Biology
|
| Street address |
60, Biopolis street
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE25212 |
Effects of Evi1 knockdown and overexpression in SKOV-3 ovarian carcinoma cells |
| GSE25213 |
EVI1 and AP1 Interact to Transcriptionally Regulate a Feed-Forward Loop Important for Cancer Cell Proliferation and Adhesion |
|
| Relations |
| Reanalyzed by |
GSE86357 |
