|
| Status |
Public on Mar 30, 2012 |
| Title |
Neurons at 24h_untreated |
| Sample type |
RNA |
| |
|
| Source name |
Cerebellar neurons from newborns, untreated, cultured for 24 hours
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Primary culture of cerebellar neurons
age: Prepared from P4-P7 pups
genotype: Wild-type
|
| Treatment protocol |
Cultures were either treated with 10-7 M thyroid hormone (T3) for 6, 16, 24 or 48 hours or left untreated the same amount of time as control cultures.
|
| Growth protocol |
Cerebellar neurons were retrieved from newborns by mechanic dissociation, plated in serum-free medium for 48 hours and then treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy micro-kit and quality was assessed by Agilent BioAnalyzer
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the two-cycle cDNA synthesis Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChip were scanned using an Affymetrix Genechip 3000 7G scanner.
|
| Description |
Gene expression data from cerebellar neurons culture 24h, untreated
|
| Data processing |
Data were analyzed using Affymetrix GeneChip Operating Software (GCOS) and Microarray Suite version 5.0 (MAS5.0) softwares and results were pairwise compared to extract up- and down-regulated genes.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Mar 30, 2012 |
| Contact name |
Fabrice Chatonnet |
| E-mail(s) |
[email protected]
|
| Phone |
+33 2 99 28 99 39
|
| Organization name |
University Hospital of Rennes
|
| Lab |
Inserm U1236 MICMAC
|
| Street address |
2 rue Henri le Guilloux
|
| City |
Rennes |
| ZIP/Postal code |
35033 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE24793 |
Target genes of thyroid hormone in cerebellum neurons |
|
