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Sample GSM606218 Query DataSets for GSM606218
Status Public on Dec 29, 2011
Title 4041-1 E2F3 Heterozygous
Sample type RNA
 
Source name E2F3 Heterozygous
Organism Mus musculus
Characteristics strain: FVB
genotype: E2F3 heterozygous
tissue: mammary gland tumor
Treatment protocol Tumors were flash frozen.
Growth protocol All tumors are from MMTV-Myc transgenics in an FVB background.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from mouse mammary tumors using the Qiagen RNeasy Midi Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix Mouse 430A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 4041-1
Data processing The data were processed using Affymetrix Expression Console to generate MAS5 data.
 
Submission date Oct 08, 2010
Last update date Dec 29, 2011
Contact name Eran Andrechek
Phone 517-884-5042
Organization name Michigan State University
Department Physiology
Lab Andrechek
Street address 2120 BPS Building
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
 
Platform ID GPL8321
Series (1)
GSE24594 MMTV-Myc tumor development in E2F-null backgrounds

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 386.0524
AFFX-BioB-M_at 538.4212
AFFX-BioB-3_at 361.2111
AFFX-BioC-5_at 982.7905
AFFX-BioC-3_at 1627.905
AFFX-BioDn-5_at 2355.051
AFFX-BioDn-3_at 5139.132
AFFX-CreX-5_at 13763.72
AFFX-CreX-3_at 13897.17
AFFX-DapX-5_at 1963.315
AFFX-DapX-M_at 2993.549
AFFX-DapX-3_at 3974.852
AFFX-LysX-5_at 189.1909
AFFX-LysX-M_at 282.9461
AFFX-LysX-3_at 670.0208
AFFX-PheX-5_at 284.8566
AFFX-PheX-M_at 328.5813
AFFX-PheX-3_at 431.4106
AFFX-ThrX-5_at 468.4578
AFFX-ThrX-M_at 623.709

Total number of rows: 22690

Table truncated, full table size 455 Kbytes.




Supplementary file Size Download File type/resource
GSM606218.CEL.gz 2.0 Mb (ftp)(http) CEL
GSM606218.CHP.gz 202.3 Kb (ftp)(http) CHP
Processed data included within Sample table

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