|
| Status |
Public on Mar 31, 2011 |
| Title |
Control, 293TetLuc, doxcycline 48 h, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
293 luciferase inducible line, doxycycline 48 h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: embryonic kidney cell
cell line: 293TetLuc
immortalization method: transformed with sheared Ad5 DNA
|
| Treatment protocol |
Cells were treated with doxycycline (50 nanogram/ml) for 48 h.
|
| Growth protocol |
Cells were grown to log-phase in DMEM supplemented with 10% tetracycline-free fetal bovine serum before induction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a RNeasy Mini kit (Qiagene), according to the manufacturer’s instructions. A 2-round amplification was performed with the ExpressArt TRinucleotide mRNA Amplification Kit (AmpTec) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Gene 1.0 ST Arrays (Affymetrix) and scanned at the NHRI Microarray Core Facility (Miaoli County, Taiwan).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
| Description |
Cell densitiy was maintained at 200,000 / ml before induction.
|
| Data processing |
The data were analyzed using Affymetrix Expression Console™ Software (version 1.1) default analysis settings and RMA (Robust Multi-Array) summarization and normalization options.
|
| |
|
| Submission date |
Oct 07, 2010 |
| Last update date |
Mar 31, 2011 |
| Contact name |
Su-Fang Lin |
| E-mail(s) |
[email protected]
|
| Organization name |
National Health Research Institutes, Taiwan
|
| Department |
NICR
|
| Lab |
R2-1211
|
| Street address |
No 35, Keyan Road, Zhunan Town
|
| City |
Miaoli Conunty |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE24585 |
Expression profiling of host genes modulated by Epstein-Barr virus (EBV) Rta in HEK293 cells |
| GSE24587 |
Epstein–Barr virus (EBV) Rta-mediated cell cycle arrest enables permissive replication of EBV and Kaposi’s sarcoma-associated herpesvirus in 293 cells |
|
