|
| Status |
Public on Feb 12, 2011 |
| Title |
TetR-IE1_uninduced_replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Cells with Tet repressor and Tet-inducible hCMV IE1, uninduced
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human embryonic lung fibroblasts
cell line: MRC-5
inducible protein: hCMV (Towne strain) 72-kDa IE1
|
| Treatment protocol |
Growth-arrested (confluent) cells were induced by addition of 1 µg/ml doxycycline to the culture media for 24 h or 72 h before RNA preparation
|
| Growth protocol |
Cells were maintained in Dulbecco's Modified Eagle’s Medium (Invitrogen) supplemented with 10% fetal calf serum, 100 units/ml penicillin, and 100 µg/ml streptomycin at 37°C and 5% CO2 in a humidified incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol (Invitrogen) and Phase Lock Gel Heavy (Eppendorf) and further purified using the RNeasy Mini Kit (Qiagen) including on-column DNase digestion according to the manufacturers' instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated sense strand DNA was prepared from 100 ng total RNA using reagents and protocols specified in the GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual (Affymetrix, P/N 701880 Rev. 4)
|
| |
|
| Hybridization protocol |
Standard reagents and protocols from Affymetrix were used for hybridization
|
| Scan protocol |
For image acquisition, an Agilent G2565BA Microarray Scanner was used
|
| Description |
Gene expression data
|
| Data processing |
The Robust Multi-Array Average (RMA) algorithm was used for creation of the summarized probe intensity signals, and data files were generated by the Affymetrix GeneChip Operating 1.4 Software
|
| |
|
| Submission date |
Sep 29, 2010 |
| Last update date |
Feb 12, 2011 |
| Contact name |
Michael Nevels |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Regensburg
|
| Department |
Medical Microbiology
|
| Lab |
Nevels
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE24434 |
Host cell transcriptome response to expression of the human cytomegalovirus (hCMV) 72-kDa immediate-early 1 (IE1) protein |
|
