|
| Status |
Public on Feb 01, 2011 |
| Title |
CAST/EiJTCE liver sample batch 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pooled mouse tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: male
|
| Treatment protocol |
Fed male mice age 7-9 weeks were given 2100 mg/kg trichloroethylene in corn oil (10 ml/kg) by gavage or corn oil as vehicle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver using Qiagen mini kit per manufacturer's instruction. Reference samples were pooled RNA from several organs from C57Bl/6J male mouse.
|
| Label |
cy 3
|
| Label protocol |
Low RNA Input Fluuorescent Linear Amp Kit (Agilent 5184-3253) per manufacturer's instruction.
|
| |
|
| Channel 2 |
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: CAST/EiJ
treatment: TCE
gender: male
|
| Treatment protocol |
Fed male mice age 7-9 weeks were given 2100 mg/kg trichloroethylene in corn oil (10 ml/kg) by gavage or corn oil as vehicle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver using Qiagen mini kit per manufacturer's instruction. Reference samples were pooled RNA from several organs from C57Bl/6J male mouse.
|
| Label |
Cy 5
|
| Label protocol |
Low RNA Input Fluuorescent Linear Amp Kit (Agilent 5184-3253) per manufacturer's instruction.
|
| |
|
| |
| Hybridization protocol |
Aglient Hybridization kit catalog # 5190-0404 was followed per manufacturer's instruction
|
| Scan protocol |
Agilent scanner G2505A model
|
| Description |
liver sample batch 1
|
| Data processing |
Feature Extraction Agilent . Data was filtered by only accepting those genes with 70% or more values present.
sample batch normalization: Samples were run by batch with the same sample used for batch normalization by COMBAT.
|
| |
|
| Submission date |
Sep 22, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Blair Urquhart Bradford |
| E-mail(s) |
[email protected]
|
| Phone |
919-966-6343
|
| Organization name |
University of North Carolina
|
| Department |
Environmental Sciences and Engineering
|
| Lab |
Envrionmental Genomics
|
| Street address |
135 Dauer Dr. CB 7431
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7431 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE24278 |
The effect of trichloroethylene on gene expression in mouse liver |
|
