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Sample GSM596005 Query DataSets for GSM596005
Status Public on Dec 06, 2010
Title Gene expression_sex_1322UT
Sample type RNA
 
Source name Biceps Brachii
Organism Homo sapiens
Characteristics gender: female
age: young
condition: resting
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
Label Biotin
Label protocol Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized by using Enzo Bioarray High Yield RNA transcription Labeling Kit (Affymetrix).
 
Hybridization protocol Hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to the manufacturer’s instructions.
Scan protocol The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent).
Data processing The probe arrays were scanned twice and the stored images were analyzed using the Expression Console (Affymetrix, Santa Clara, CA). RMA data was derived by using Bioconductor.
 
Submission date Sep 20, 2010
Last update date Dec 06, 2010
Contact name GORDON PAUL
E-mail(s) [email protected]
Organization name University of Michigan
Street address 325 E Eisenhower
City Ann Arbor
State/province MI
ZIP/Postal code 48108
Country USA
 
Platform ID GPL570
Series (1)
GSE24235 Skeletal muscle gene expression in response to resistance exercise: sex specific regulation

Data table header descriptions
ID_REF
VALUE RMA data

Data table
ID_REF VALUE
1007_s_at 7.825169951
1053_at 4.768366689
117_at 4.082826847
121_at 6.939656532
1255_g_at 2.748783208
1294_at 4.892931866
1316_at 4.655398096
1320_at 3.812071379
1405_i_at 4.181452058
1431_at 2.878458825
1438_at 4.827587845
1487_at 8.384324733
1494_f_at 5.006480198
1552256_a_at 5.257233313
1552257_a_at 5.664733818
1552258_at 3.237239549
1552261_at 3.8825307
1552263_at 2.668768109
1552264_a_at 6.620638754
1552266_at 2.769207198

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM596005.CEL.gz 4.4 Mb (ftp)(http) CEL
GSM596005.chp.gz 392.8 Kb (ftp)(http) CHP
Processed data included within Sample table

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