|
| Status |
Public on Nov 01, 2011 |
| Title |
Healthy donor_biological replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole lung tissue_healthy donor lung
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
lung source: routine lung volume reduction
phenotype: healthy
gender: unknown
age: unknown
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with RNAqueous-4PCR kits (Ambion, Inc., Austin, TX) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA ( Affymetrix Technical Manual).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Array Scanner (7G).
|
| Description |
Normal_A
|
| Data processing |
All pre-processing and data analyses were performed using R version 2.9 and the Bioconductor packages designed for use with Affymetrix microarray data.
|
| |
|
| Submission date |
Sep 17, 2010 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Eric B. Meltzer |
| E-mail(s) |
[email protected]
|
| Phone |
919-681-5961
|
| Fax |
919-681-4637
|
| Organization name |
Duke University
|
| Department |
Department of Medicine, Division of Pulmonary, Allergy and Critical Care
|
| Street address |
DUMC 102355
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE24206 |
Validated Gene Expression Signatures of Idiopathic Pulmonary Fibrosis |
|
| Relations |
| Reanalysis of |
GSM252104 |
