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GEO help: Mouse over screen elements for information. |
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Status |
Public on Mar 30, 2023 |
Title |
INV_CNCC_4C_VP6_Rep1 |
Sample type |
SRA |
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Source name |
CNCC derived from edited H9 hESC
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Organism |
Homo sapiens |
Characteristics |
tissue: CNCC derived from edited H9 hESC genotype: Hemizygous inversion differentiation day: d5 4c viewpoint: Viewpoint 6
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Treatment protocol |
Gene editing by nucleofection, CNCC derivation as in Leung, A.W., Murdoch, B., Salem, A.F., Prasad, M.S., Gomez, G.A., and García-Castro, M.I. (2016). WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143, 398–410.
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Growth protocol |
Cultured in Essential 8 media (Gibco, A1517001) on Matrigel substrate (Corning, 354277)
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Extracted molecule |
genomic DNA |
Extraction protocol |
as in GSE98251
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Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
NextSeq 550 |
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Description |
4C-seq from CNCCs derived from H9 hESCs with an inversion on Chr7 due to CRISPR/Cas9 editing
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Data processing |
Library strategy: 4C as in GSE98251 and accompanying manuscript
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Submission date |
Mar 10, 2022 |
Last update date |
Mar 30, 2023 |
Contact name |
Justin Cotney |
Organization name |
UConn Health
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Department |
Genetics and Genome Sciences
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Street address |
400 Farmington Avenue
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City |
Farmington |
State/province |
CT |
ZIP/Postal code |
06030-6403 |
Country |
USA |
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Platform ID |
GPL21697 |
Series (1) |
GSE198297 |
Chromatin state and chromatin architecture in mouse embryonic craniofacial tissue, neural crest cell culture model and human primary craniofacial tissue |
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Relations |
BioSample |
SAMN26559501 |
SRA |
SRX14427372 |
Supplementary file |
Size |
Download |
File type/resource |
GSM5944474_vp6_INV_CNCC1.bedgraph.gz |
2.0 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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