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Sample GSM5944474 Query DataSets for GSM5944474
Status Public on Mar 30, 2023
Title INV_CNCC_4C_VP6_Rep1
Sample type SRA
 
Source name CNCC derived from edited H9 hESC
Organism Homo sapiens
Characteristics tissue: CNCC derived from edited H9 hESC
genotype: Hemizygous inversion
differentiation day: d5
4c viewpoint: Viewpoint 6
Treatment protocol Gene editing by nucleofection, CNCC derivation as in Leung, A.W., Murdoch, B., Salem, A.F., Prasad, M.S., Gomez, G.A., and García-Castro, M.I. (2016). WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143, 398–410.
Growth protocol Cultured in Essential 8 media (Gibco, A1517001) on Matrigel substrate (Corning, 354277)
Extracted molecule genomic DNA
Extraction protocol as in GSE98251
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model NextSeq 550
 
Description 4C-seq from CNCCs derived from H9 hESCs with an inversion on Chr7 due to CRISPR/Cas9 editing
Data processing Library strategy: 4C
as in GSE98251 and accompanying manuscript
 
Submission date Mar 10, 2022
Last update date Mar 30, 2023
Contact name Justin Cotney
Organization name UConn Health
Department Genetics and Genome Sciences
Street address 400 Farmington Avenue
City Farmington
State/province CT
ZIP/Postal code 06030-6403
Country USA
 
Platform ID GPL21697
Series (1)
GSE198297 Chromatin state and chromatin architecture in mouse embryonic craniofacial tissue, neural crest cell culture model and human primary craniofacial tissue
Relations
BioSample SAMN26559501
SRA SRX14427372

Supplementary file Size Download File type/resource
GSM5944474_vp6_INV_CNCC1.bedgraph.gz 2.0 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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