|
| Status |
Public on Feb 23, 2022 |
| Title |
CD90-EPCR- MPP 11 |
| Sample type |
SRA |
| |
|
| Source name |
cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cord blood CD34+CD38-CD45RA-CD90-EPCR- cells
age: post-natal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using RNeasy Plus Micro Kit (Qiagen) and quality was verified in an Agilent 2100 Bioanalyzer (Agilent).
KAPA Stranded with RiboErase RNA-seq kit (Kapa Biosystems).
RNA-seq 30 million single-end 100 bp reads/sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
AFO296A20
mRNA
|
| Data processing |
Raw sequencing reads were mapped with STAR.
Expression counts estimates and transcripts per million (TPM) were generated using RSEM.
Genome_build: GRCh38
Supplementary_files_format_and_content: counts_bulk_MPP_ENS.csv: Raw counts in comma-separated matrix format.
|
| |
|
| Submission date |
Feb 20, 2022 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE197079 |
Newly defined human hematopoietic stem cell populations |
|
| Relations |
| BioSample |
SAMN26106886 |
| SRA |
SRX14235280 |
