|
| Status |
Public on Feb 21, 2011 |
| Title |
Transgenic ER-FUS3, 48h, ESTR, 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ER-FUS3 transgenic plant (Col-0), untreated
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: no treatment
treatment time: 0h
replicate: 1 of 2
|
| Growth protocol |
Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Linear Amplification Kit.
|
| |
|
| Channel 2 |
| Source name |
ER-FUS3 transgenic plant (Col-0), ESTR, 48h
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype: ER-FUS3 transgenic
tissue: seedling
age: 7 days
treatment: 4μM estrogen (ESTR)
treatment time: 48h
replicate: 1 of 2
|
| Growth protocol |
Seeds of an ER8-FUS3 transgenic line were plated on 0.6% agar plates with the same composition as below and kept for 3 days at 4°C, then shifted to 22°C under continuous light, cultured for 4 days. The transgenic plant seedlings with expanded cotyledons were transferred from the plates to a 10 ml liquid medium [half-strength MS salt mixture, pH 5.7, B5 vitamins, and 1% (w/v) sucrose] in a 100 ml Erlenmyer flask (100 seedlings per flask), cultured for additional 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted by RNeasy Mini Kit (Qiagen) and was purified again using the same kit.
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Linear Amplification Kit.
|
| |
|
| |
| Hybridization protocol |
Agilent Arabidopsis2 oligo microarray hybridization protocol.
|
| Scan protocol |
Dual-laser Microarray scanner Agilent Technologies.
|
| Description |
ER-FUS3 transgenic plants were used to identify the genes up-regulated in young seedlings of Arabidopsis in response to ectopic expression of FUS3.
|
| Data processing |
Agilent Feature Extraction software version A.6.1.1.
|
| |
|
| Submission date |
Sep 03, 2010 |
| Last update date |
Feb 21, 2011 |
| Contact name |
Akiko Yamamoto |
| E-mail(s) |
[email protected]
|
| Organization name |
Nagoya University
|
| Department |
Bioscience and Biotechnology Center
|
| Lab |
Laboratory of Plant Cell Function
|
| Street address |
Furo-cho
|
| City |
Nagoya |
| ZIP/Postal code |
464-8601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL888 |
| Series (1) |
| GSE23974 |
Gene regulation by the Arabidopsis seed maturation master regulator FUS3 |
|
