|
| Status |
Public on May 16, 2022 |
| Title |
sodh_starved_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
whole larvae, third instar
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: sodh1, sodh2 mutant
agent: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Larvae were flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Standard mRNA Library Prep Kit LT was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
sodh_sorbitol.diff, sodh_fructose.diff
|
| Data processing |
RNA-seq reads were mapped to the FlyBase reference genome (Dmel Release 6.19) using Tophat2.
Transcript abundance and splice variant identification were determined using Cufflinks.
Differential expression analysis was performed using CuffDiff.
Genome_build: Dmel 6.19
Supplementary_files_format_and_content: diff file from Cuffdiff
|
| |
|
| Submission date |
Jan 31, 2022 |
| Last update date |
May 16, 2022 |
| Contact name |
Hiroko Sano |
| E-mail(s) |
[email protected]
|
| Organization name |
Kurume University
|
| Street address |
67 Asahi-machi
|
| City |
Kurume |
| State/province |
Fukuoka |
| ZIP/Postal code |
8300011 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE195759 |
Quantitative analysis of sugar-induced gene expression in Drosophila larvae |
|
| Relations |
| BioSample |
SAMN25512568 |
| SRA |
SRX13993154 |
