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| Status |
Public on Jan 09, 2022 |
| Title |
NBRC1958 at 60 g/L succinic acid |
| Sample type |
RNA |
| |
|
| Source name |
Saccharomyces cerevisiae NBRC1958 in the YPD medium and 60 g/L succinic acid was supplemented
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: Saccharomyces cerevisiae NBRC1958
agent: 60 g/L succinic acid
|
| Growth protocol |
at 35℃, 200 rpm growth for 6 hours, with different succinic acid supplement (0-60 g/L).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Ambion #1792 cRNA扩增标记试剂盒
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Saccharomyces cerevisiae Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450/250.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000.
|
| Description |
Gene expression data from Saccharomyces cerevisiae NBRC1958 and fermented with 60 g/L succinic acid .
|
| Data processing |
The data were analyzed with Microarray Suite version 3.0 (MAS 3.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
q-value ≤ 5% and Fold Change ≥2 or≤0.5
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| |
|
| Submission date |
Jan 07, 2022 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Ranran Su |
| Organization name |
SiChuan University
|
| Street address |
Chuanda Road
|
| City |
Chengdu |
| ZIP/Postal code |
610044 |
| Country |
China |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE193190 |
Expression data from Saccharomyces cerevisiae |
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