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Status |
Public on Nov 29, 2022 |
Title |
Person C_CD4_IFNg, 24h |
Sample type |
SRA |
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Source name |
Primary activated CD4 cells from PBMC
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Organism |
Homo sapiens |
Characteristics |
cell type: activated_CD4 agent: IFNg time point: 24h treatment: 0 ng/ml YM155 person: C
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Treatment protocol |
Treated with survivin inhibitor YM155 (0 or 10 ng/ml), IFNg last 2 h
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Growth protocol |
Lymphoprep separation of PBMC, Magnetic separation of CD4 cells, culture activated with anti-CD3, 24h total
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA purification (Norgen, Ontario, Canada)
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Description |
2C_CD4_IFNg
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Data processing |
Aligned: STAR, Ref.genome: Homo_sapiens.GRCh38.dna.primary_assembly.fa (Ensembl), quantification: featureCounts Genome_build: HG38 Supplementary_files_format_and_content: matrix with ENS number and samples in each one column
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Submission date |
Dec 07, 2021 |
Last update date |
Nov 29, 2022 |
Contact name |
Maria I Bokarewa |
E-mail(s) |
[email protected]
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Organization name |
Gothenburg University
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Department |
Rheumatology and Inflammation Research
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Lab |
Bokarewa
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Street address |
Guldhedagatan 10A
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City |
Gothenburg |
State/province |
VG |
ZIP/Postal code |
SE41346 |
Country |
Sweden |
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Platform ID |
GPL11154 |
Series (2) |
GSE190352 |
RNAseq of CD4 T cells treated with survivivin inhibitor YM155 for 24h |
GSE190356 |
CD4 T cells |
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Relations |
BioSample |
SAMN23721563 |
SRA |
SRX13339492 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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