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Sample GSM5720185 Query DataSets for GSM5720185
Status Public on Nov 29, 2022
Title Person C_CD4_IFNg, 24h
Sample type SRA
 
Source name Primary activated CD4 cells from PBMC
Organism Homo sapiens
Characteristics cell type: activated_CD4
agent: IFNg
time point: 24h
treatment: 0 ng/ml YM155
person: C
Treatment protocol Treated with survivin inhibitor YM155 (0 or 10 ng/ml), IFNg last 2 h
Growth protocol Lymphoprep separation of PBMC, Magnetic separation of CD4 cells, culture activated with anti-CD3, 24h total
Extracted molecule total RNA
Extraction protocol Total RNA purification (Norgen, Ontario, Canada)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description 2C_CD4_IFNg
Data processing Aligned: STAR, Ref.genome: Homo_sapiens.GRCh38.dna.primary_assembly.fa (Ensembl),
quantification: featureCounts
Genome_build: HG38
Supplementary_files_format_and_content: matrix with ENS number and samples in each one column
 
Submission date Dec 07, 2021
Last update date Nov 29, 2022
Contact name Maria I Bokarewa
E-mail(s) [email protected]
Organization name Gothenburg University
Department Rheumatology and Inflammation Research
Lab Bokarewa
Street address Guldhedagatan 10A
City Gothenburg
State/province VG
ZIP/Postal code SE41346
Country Sweden
 
Platform ID GPL11154
Series (2)
GSE190352 RNAseq of CD4 T cells treated with survivivin inhibitor YM155 for 24h
GSE190356 CD4 T cells
Relations
BioSample SAMN23721563
SRA SRX13339492

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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