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Sample GSM565677 Query DataSets for GSM565677
Status Public on Dec 17, 2010
Title YPS163 MATa 30 min vs T0 - 5% EtOH #2
Sample type RNA
 
Channel 1
Source name YPS163 MATa 30 min after 5% EtOH shock
Organism Saccharomyces cerevisiae
Characteristics genotype: YPS163 MATa
protocol: YPS163 MATa 30 min after 5% EtOH shock
Treatment protocol For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
Growth protocol All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
Extracted molecule total RNA
Extraction protocol Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
Label Oyster 550 cyanine dye
Label protocol Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
 
Channel 2
Source name YPS163 MATa log growth
Organism Saccharomyces cerevisiae
Characteristics genotype: YPS163 MATa
protocol: YPS163 MATa log growth
Treatment protocol For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
Growth protocol All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
Extracted molecule total RNA
Extraction protocol Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
Label Oyster cyanine 650 dye
Label protocol Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
 
 
Hybridization protocol As indicated in Gasch AP Methods in Enzymology 350: 393-414
Scan protocol Arrays were scanned on an Axon 4000B scanner
Description 30 min EtOH shock in YPS163 MATa
Data processing Data were extracted using GenePix 6.0. Data were normalized by regional mean-centering as described by Lyne et al. 2003 BMC Genomics
 
Submission date Jul 12, 2010
Last update date Dec 17, 2010
Contact name Jeffrey A. Lewis
E-mail(s) [email protected]
Phone 608-347-3661
Organization name University of Arkansas
Department Biological Sciences
Lab Lewis Lab
Street address 850 W. Dickson St.
City Fayetteville
State/province AR
ZIP/Postal code 72701
Country USA
 
Platform ID GPL5915
Series (1)
GSE22904 Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains

Data table header descriptions
ID_REF
VALUE normalized log2 expression ratios: positive log2 value = induced by ethanol shock (or higher expressed in non-lab strain relative to DBY8268), and a negative log2 value = repressed by ethanol shock (or expressed lower in non-lab strain relative to DBY8268)

Data table
ID_REF VALUE
Q0010 null
Q0017 0.91
Q0032 null
Q0045 0.77
Q0050 null
Q0055 0.17
Q0060 null
Q0065 -0.27
Q0070 0.01
Q0075 -0.08
Q0080 1.45
Q0085 1.42
Q0092 0.41
Q0105 0.8
Q0110 -0.08
Q0115 -0.43
Q0120 null
Q0130 1.21
Q0140 null
Q0142 0.9

Total number of rows: 6306

Table truncated, full table size 82 Kbytes.




Supplementary file Size Download File type/resource
GSM565677_yg12-38a.gpr.gz 1.1 Mb (ftp)(http) GPR
Processed data included within Sample table

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