|
Status |
Public on Dec 17, 2010 |
Title |
YPS163 MATa 30 min vs T0 - 5% EtOH #2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
YPS163 MATa 30 min after 5% EtOH shock
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype: YPS163 MATa protocol: YPS163 MATa 30 min after 5% EtOH shock
|
Treatment protocol |
For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
|
Growth protocol |
All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
Label |
Oyster 550 cyanine dye
|
Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
|
|
Channel 2 |
Source name |
YPS163 MATa log growth
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype: YPS163 MATa protocol: YPS163 MATa log growth
|
Treatment protocol |
For ethanol-shocked cells, ethanol was added to a final concentration of 5% (v/v).
|
Growth protocol |
All cells were grown for at least 8 doublings in log phase in YPD medium at 30C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Hot-phenol RNA extraction as described in Gasch AP Methods in Enzymology 350: 393-414
|
Label |
Oyster cyanine 650 dye
|
Label protocol |
Indirect labeling using amino-allyl-dUTP and cyanine dyes from Flownamics (Madison, Wi) as described in Gasch AP Methods in Enzymology 350: 393-414
|
|
|
|
Hybridization protocol |
As indicated in Gasch AP Methods in Enzymology 350: 393-414
|
Scan protocol |
Arrays were scanned on an Axon 4000B scanner
|
Description |
30 min EtOH shock in YPS163 MATa
|
Data processing |
Data were extracted using GenePix 6.0. Data were normalized by regional mean-centering as described by Lyne et al. 2003 BMC Genomics
|
|
|
Submission date |
Jul 12, 2010 |
Last update date |
Dec 17, 2010 |
Contact name |
Jeffrey A. Lewis |
E-mail(s) |
[email protected]
|
Phone |
608-347-3661
|
Organization name |
University of Arkansas
|
Department |
Biological Sciences
|
Lab |
Lewis Lab
|
Street address |
850 W. Dickson St.
|
City |
Fayetteville |
State/province |
AR |
ZIP/Postal code |
72701 |
Country |
USA |
|
|
Platform ID |
GPL5915 |
Series (1) |
GSE22904 |
Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains |
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