|
| Status |
Public on Jul 12, 2010 |
| Title |
Undifferentiated Embryonal stem cells 129SV Biological Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Undifferentiated Embryonal stem cells 129SV
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: 129SV
stem cells: undifferentiated
cell type: Undifferentiated Embryonal stem cells 129SV
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen) isolation according to the manufactures standard protocol, Subsequent DNase I treatment
|
| Label |
biotin
|
| Label protocol |
Labeling protocol was carried out with 300 ng of purified RNA, with biotin, accord to Affymetrix Gene Mouse Arrays 1.0 ST protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 mg of biotinilated RNA were hybridized for 16 hr at 45C into individual Affymetrix Gene Mouse Arrays 1.0 ST. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix) according to GeneChip® Whole Transcript (WT) Sense Target Labeling Assay manual (Affymetrix) instructions.
|
| Scan protocol |
Microarrays were scanned in a GeneChip® Scanner 3000 7G (Affymetrix) and quality control was evaluated by Affymetrix software (Expression ConsoleTM).
|
| Description |
ES_RI_R2
|
| Data processing |
The data where preprocessed (RMA) using the Expression Console TM from Affymetrix.
|
| |
|
| Submission date |
Jun 28, 2010 |
| Last update date |
Jul 09, 2010 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
[email protected]
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22605 |
Pluripotent embryonic stem cells and multipotent adult germline stem cells reveal similar transcriptomes including pluripotency-related genes |
|
