|
| Status |
Public on Jun 25, 2010 |
| Title |
Normal B Cell Control 5 Jel037U133B |
| Sample type |
RNA |
| |
|
| Source name |
Purified B cells from peripheral blood from donors 60 years of age and older
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: normal
cell type: Purified B cells from peripheral blood from donors 60 years of age and older
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation by the Trizol method following manufacturer's protocol from purified B cells
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Enzo, New York, NY).
|
| |
|
| Hybridization protocol |
15ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human U133 A and B GeneChips® . GeneChips were washed and stained following the Expression Analysis Technical Manual, Affymetrix, Santa Clara, CA
|
| Scan protocol |
Hybridized arrays were scanned using a GeneChip® 3000 scanner
|
| Description |
Gene expression data from peripheral blood B cells age matched normal controls
|
| Data processing |
Affymetrix GeneChip® Operating System software v.1.3 (Santa Clara, CA) were used to quantitatively analyze the scanned image
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Jun 24, 2010 |
| Contact name |
Brian Bot |
| Organization name |
Mayo Clinic
|
| Street address |
200 1st Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE22529 |
Gene expression profiles in CLL |
|
