|
| Status |
Public on Sep 20, 2021 |
| Title |
atg5 control, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
atg5 control
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: atg5
treatment: control
tissue: leaf
|
| Treatment protocol |
Whole Arabidopsis plants (4-week-old) were gently submerged at depths of 10 cm beneath the water surface under normal light/dark cycle condition. Plant samples for microarray were collected after 0 h or 48 h submergence treatment.
|
| Growth protocol |
plants were grown under 12 h L/ 12 h D for 28 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Plant Mini kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
standard protocol
|
| |
|
| Hybridization protocol |
standard protocol
|
| Scan protocol |
standard protocol
|
| Description |
atg5_0h1
|
| Data processing |
Affymetrix ATH1 Arabidopsis chips (Affymetrix) were used for labeling, hybridization, scanning, and detection. Affymetrix Gene Chip software MAS 5.0 and GeneSpring 12.6 (Agilent) were applied for raw data collection. Raw data were processed by R packages affy, and normalized with gcRMA method.
|
| |
|
| Submission date |
Sep 17, 2021 |
| Last update date |
Sep 21, 2021 |
| Contact name |
mk yang |
| E-mail(s) |
[email protected]
|
| Organization name |
scau
|
| Street address |
wushan
|
| City |
guangzhou |
| ZIP/Postal code |
510642 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE184340 |
Studies of autophagy pathway during submergence in Arabidopsis thaliana |
|
