|
| Status |
Public on Oct 06, 2022 |
| Title |
WT_switch_gut_3 |
| Sample type |
SRA |
| |
|
| Source name |
Adult female gut
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: S106
Sex: female
age: 30 days
induction: RU486+ 3weeks, then RU- 1 week
|
| Treatment protocol |
Mated 48h, separated from males, fed 200 µM RU486 for 21 days, and switch to control food for 7 days.
|
| Growth protocol |
Flies reared on 10% yeast, 5% sucrose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Gut and fat body dissection, Trizol-chloroform extraction, NextFlex Poly(A) beads (PerkinElmer NOVA-512981) for polyA enrichment.
NextFlex Rapid Directional qRNAseq v2 reagents, (barcode sets C and D, PerkinElmer NOVA-5130-14 and NOVA-5130-15 ) and 16 cycles of PCR.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
22_S3
|
| Data processing |
Read quality assessed with FastQC
Aligned to D. melanogaster genome by HiSAT2, sorted by Samtools
Counted with featurecounts
Analysed with DESeq2
Genome_build: dmel 6.19
Supplementary_files_format_and_content: processedData_foxo_switch_RNA.xlsx: Excel file containing read counts for genes analysed in gut and fat body.
|
| |
|
| Submission date |
Sep 06, 2021 |
| Last update date |
Oct 06, 2022 |
| Contact name |
MengJia Li |
| E-mail(s) |
[email protected]
|
| Phone |
07763753347
|
| Organization name |
University College London
|
| Street address |
flat38 midhopse house, midhope street
|
| City |
LONDON |
| State/province |
Please Select |
| ZIP/Postal code |
WC1H 8HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE183541 |
Transcriptional memory of dFOXO activity in early adulthood delays late life mortality [RNA-seq] |
| GSE183542 |
Transcriptional memory of dFOXO activity in early adulthood delays late life mortality. |
|
| Relations |
| BioSample |
SAMN21245618 |
| SRA |
SRX12027479 |
