|
| Status |
Public on Aug 01, 2021 |
| Title |
EV-3 |
| Sample type |
genomic |
| |
|
| Source name |
Prostate
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Pca cell line
passage: 20-30
genotype/variation: SPOP WT
|
| Treatment protocol |
22Rv1 cells were infected with lentivirus expressiong empty vector or SPOP F102C construct and cultured for 1 week to stably express SPOP F102C protein.
|
| Growth protocol |
Cells were maintained at 37°C and 5% CO2 in RPMI 1640 containing 10% fetal bovine serum (FBS) and 1% antibiotic/antimycotic (Thermo Fisher Scientific).FGF-2 (NS expansion medium).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA were extracted using standard DNA extraction method
|
| Label |
cy3, cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium 850K Infinium® MethylationEPIC Beadchip using standard Illumina protocol at the University of Minnesota Genomics Center.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader Hi-Scan system.
|
| Data processing |
Resultant IDAT files were processed and normalized using Illumina's GenomeStudio software (version 2011.1).
value definition: Average Beta
|
| |
|
| Submission date |
Jun 30, 2021 |
| Last update date |
Aug 01, 2021 |
| Contact name |
LIGUO WANG |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Department |
Division of Computational Biology
|
| Street address |
200 1st St SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE179234 |
SPOP mutations derived from prostate cancer establish a DNA hypermethylation epigenome through stabilizing GLP histone methyltransferase [methylation array] |
|
