|
| Status |
Public on Aug 23, 2021 |
| Title |
GEX_IFNgDay17_2 [GEX_04] |
| Sample type |
SRA |
| |
|
| Source name |
BM
|
| Organism |
Mus musculus |
| Characteristics |
treatment: IFNγ_treated
timepoint: Day17
celltype: BM
|
| Treatment protocol |
lineage negative transduced cells transplant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
BM cells were sorted for viability (Annexin V) and split in B cell and non-B cells: one gate to collect CD19+ OFP+/- cells and one gate to collect CD45+CD19- cells. After sorting cells were washed and counted
Chromium(TM) Single Cell 5' Library & Gel Bead Kit and ChromiumTM Single Cell V(D)J Enrichment Kit, Mouse T Cell (10xGENOMICS) according to manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Cellranger 10X demultiplexing (mkfastq)
Cellranger 10X quantification (counts)
Genome_build: refdata-cellranger-mm10-3.1.0
Supplementary_files_format_and_content: sparse matrix (mtx)
|
| |
|
| Submission date |
Jun 25, 2021 |
| Last update date |
Aug 23, 2021 |
| Contact name |
Matteo Barcella |
| E-mail(s) |
[email protected]
|
| Organization name |
SR-TIGET
|
| Street address |
Via Olgettina 60
|
| City |
Milano |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE178941 |
Myeloid cell-based delivery of IFN-γ reprograms leukemia microenvironment and induces anti-tumoral immune responses |
|
| Relations |
| BioSample |
SAMN19876209 |
| SRA |
SRX11230611 |
