|
| Status |
Public on Dec 31, 2012 |
| Title |
IL-1b (2.5 ng/mL) treated SMCs rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat aortic smooth muscle cells treated with IL-1b (2.5 ng/mL)
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: Sprague-Dawley
cell type: aortic smooth muscle cell
agent: IL-1b
|
| Treatment protocol |
Cells treated with either vehicle (.1% BSA) or IL-1b (2.5 ng/mL) for 24 hours.
|
| Growth protocol |
Smooth muscle cells were grown in 10% serum-containing media for 5 days, then switched to serum-free media for 3 days prior to treatment for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
10 ug of cRNA was hybridized for 16 hours on Affymetrix rat 230 2.0 GeneChips and stained using Affymetrix Fluidics Station 450 according to Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Gene expression data from IL-1b-treated rat aortic smooth muscle cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and between-group fold changes were calculated using Affymetrix Data Mining Tool 3.0. Local pooled error testing was performed by the University of Virginia Gene Expression Open Source System (GEOSS) software.
|
| |
|
| Submission date |
Apr 20, 2010 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Gary Owens |
| Organization name |
University of Virginia
|
| Department |
Molecular Physiology and Biological Physics
|
| Lab |
Gary Owens' Laboratory
|
| Street address |
415 Lane Road
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE21403 |
Effect of IL-1b treatment on cultured rat aortic smooth muscle cells |
|
